Primer Designer This page/program is a modification of the Dana-Farber Cancer Institute's 'Oligo Calculator'Ciona intestinalis genome: Ghost Database UTR Remover Enter Gene Sequence in Box Enter Translated Sequence in Box Gene Sequence without UTRs Gene is Reverse Complimented Intron/Exon Constructer Enter Results From CLUSTAL 2.1 multiple sequence alignment in Box Constructed Gene Amplicon Length: Percent Deviation:% Percent Intron:% Oligo Finder Enter Exon/Intron Sequence in Box Reading Frame: 1 2 3 Melting Temp (deg C): Min: Max: %GC Content: Min: Max: Primer Length: Min: Max: Amplicon Length: +/- Maximum Intron Proportion: % Primer Root Name: Primer 1 Designation: Primer 2 Designation: Find Pair with: lowest intron base count lowest intron proportion lowest amplicon deviation Matching Primers: Matching Pairs: Best Matching Pair Primer Alignment Enter Genome Sequence in Box Extract Gene Sequence from: Gene Constructer Box Oligo Finder Box Genome Name: Gene Name: Reverse Complimented: Genome Gene Include Genome FASTA: Alignment FASTA